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BB1_23_dCAS9(3xFLAG_SV40NLS)
(Plasmid #161478)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 161478 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    BB1_L_23_syn_BsaI
  • Backbone size w/o insert (bp) 2000
  • Total vector size (bp) 6147
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCAS9
  • Alt name
    dead Cas9
  • Tag / Fusion Protein
    • FLAG Tag, SV40 NLS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BpiI (destroyed during cloning)
  • 3′ cloning site BpiI (destroyed during cloning)
  • 5′ sequencing primer CAGGAAACAGCTATGAC
  • 3′ sequencing primer GTAAAACGACGGCCAGTT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BB1_23_dCAS9(3xFLAG_SV40NLS) was a gift from Brigitte Gasser & Diethard Mattanovich (Addgene plasmid # 161478 ; http://n2t.net/addgene:161478 ; RRID:Addgene_161478)
  • For your References section:

    Fine-Tuning of Transcription in Pichia pastoris Using dCas9 and RNA Scaffolds. Baumschabl M, Prielhofer R, Mattanovich D, Steiger MG. ACS Synth Biol. 2020 Dec 18;9(12):3202-3209. doi: 10.1021/acssynbio.0c00214. Epub 2020 Nov 12. 10.1021/acssynbio.0c00214 PubMed 33180466