PurposeMammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 7 amino acids at the carboxyl end of the mEGFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||163431||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
- Promoter CMV
/ Fusion Protein
- mEGFP (C terminal on insert)
- Cloning method Gibson Cloning
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Nup54-mEGFP494(flex2) was a gift from Sanford Simon (Addgene plasmid # 163431 ; http://n2t.net/addgene:163431 ; RRID:Addgene_163431)