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Sanford Simon Lab: Simon lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose  
163417Nup133_mEGFP(-8a)Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
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163418Nup133_mEGFP(-8b)Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
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163419Nup133_mEGFP(-9a)Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)
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163420Nup133_mEGFP(-9b)Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)
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163424Nup58_mEGFP(-7)Mammalian expression of Nup58 with a mEGFP (missing the first 7 amino acids) at amino acid position 412
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163426Nup54-mEGFP494(0)Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of the mEGFP
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163427Nup54-mEGFP494(1)Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of the mEGFP
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163428Nup54-mEGFP494(2)Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of the mEGFP
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163429Nup54-mEGFP494(flex0)Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 5 amino acids at the carboxyl end of the mEGFP
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163430Nup54-mEGFP494(flex1)Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 6 amino acids at the carboxyl end of the mEGFP
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163431Nup54-mEGFP494(flex2)Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 7 amino acids at the carboxyl end of the mEGFP
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163432Nup54_mEGFP494(-4)Mammalian expression of Nup54 with a mEGFP (missing the first 4 amino acids) at amino acid position 494
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163433Nup54_mEGFP494(-5)Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494
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163434Nup54_mEGFP494(-6)Mammalian expression of Nup54 with a mEGFP (missing the first 6 amino acids) at amino acid position 494
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163438BFP-RanQ69LMammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredation
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163443NLS-tdTomatoBacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backbone
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Recombinant Antibodies from Article