pCS2z-lmo2
(Plasmid #164657)

• Purpose: for the synthesis of zebrafish lmo2 mRNA
• Depositing Lab: Didier Stainier
• Publication: mRNA expression plasmids for npas4l, etsrp, tal1 and lmo2 (unpublished)

Backbone

• Vector backbone: pCS2z
  (Search Vector Database)
• Backbone manufacturer: addgene #62214
• Backbone size w/o insert (bp): 4292
• Total vector size (bp): 4772
• Vector type: mRNA synthesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lmo2
• Species: D. rerio (zebrafish)
• Insert Size (bp): 480
• Entrez Gene: lmo2 (a.k.a. wu:fc83a08, zgc:111930)
• Promoter: SP6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • pCS2z-lmo2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry