AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)
(Plasmid
#165456)
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PurposeInducible ZEB2 expression from AAVS1 locus
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Depositing Lab
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Depositing OrganizationMRC Laboratory of Molecular Biology
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 165456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 165456-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 165456-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneAAVS1-Puro
- Backbone size w/o insert (bp) 11520
- Total vector size (bp) 15160
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameZEB2
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Alt nameSIP1
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SpeciesH. sapiens (human)
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Insert Size (bp)3641
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GenBank IDNM_014795.3
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Entrez GeneZEB2 (a.k.a. HSPC082, SIP-1, SIP1, SMADIP1, ZFHX1B)
- Promoter CAG
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site FseI (not destroyed)
- 5′ sequencing primer CGGCGCGAACTGGATCCAC
- (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byThe ZEB2 was purchased as an ultimate ORF clone in the pENTRY221 (Thermo Scientific, #IOH53645).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 165456-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 165456-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A) was a gift from Madeline Lancaster (Addgene plasmid # 165456 ; http://n2t.net/addgene:165456 ; RRID:Addgene_165456) -
For your References section:
An early cell shape transition drives evolutionary expansion of the human forebrain. Benito-Kwiecinski S, Giandomenico SL, Sutcliffe M, Riis ES, Freire-Pritchett P, Kelava I, Wunderlich S, Martin U, Wray GA, McDole K, Lancaster MA. Cell. 2021 Apr 15;184(8):2084-2102.e19. doi: 10.1016/j.cell.2021.02.050. Epub 2021 Mar 24. 10.1016/j.cell.2021.02.050 PubMed 33765444
Map uploaded by the depositor.
