|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16576||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepShuttle / pAdTrack ?
Backbone manufacturerVogelstein Lab
Vector typeMammalian Expression, Adenoviral
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
Insert Size (bp)3354
Entrez GeneAPC (a.k.a. BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46)
/ Fusion Protein
- EGFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site ApaLI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The cAPC containing amino acids 958-2075 (nucleotides 2890–6240) was isolated from pCMV-APC by BglII digestion. This fragment was subcloned into the pEGFP-C1 (Clontech). The cassette containing the EGFP-tagged cAPC was further subcloned into the shuttle vector (pShuttle) using Apal I and Mlu I sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Ad-CBR (GFP/cAPC) was a gift from Bert Vogelstein (Addgene plasmid # 16576 ; http://n2t.net/addgene:16576 ; RRID:Addgene_16576)
For your References section:The beta-catenin binding domain of adenomatous polyposis coli is sufficient for tumor suppression. Shih IM, Yu J, He TC, Vogelstein B, Kinzler KW. Cancer Res. 2000 Mar 15. 60(6):1671-6. PubMed 10749138
Map uploaded by the depositor.