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Ad-CBR (GFP/cAPC)
(Plasmid #16576)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16576 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pShuttle / pAdTrack ?
  • Backbone manufacturer
    Vogelstein Lab
  • Vector type
    Mammalian Expression, Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    cAPC
  • Alt name
    APC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3354
  • Mutation
    Residues 958-2076
  • Entrez Gene
    APC (a.k.a. BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaLI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The cAPC containing amino acids 958-2075 (nucleotides 2890–6240) was isolated from pCMV-APC by BglII digestion. This fragment was subcloned into the pEGFP-C1 (Clontech). The cassette containing the EGFP-tagged cAPC was further subcloned into the shuttle vector (pShuttle) using Apal I and Mlu I sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ad-CBR (GFP/cAPC) was a gift from Bert Vogelstein (Addgene plasmid # 16576 ; http://n2t.net/addgene:16576 ; RRID:Addgene_16576)
  • For your References section:

    The beta-catenin binding domain of adenomatous polyposis coli is sufficient for tumor suppression. Shih IM, Yu J, He TC, Vogelstein B, Kinzler KW. Cancer Res. 2000 Mar 15. 60(6):1671-6. PubMed 10749138