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SARS CoV-2 HexaPro spike (bio-His)
(Plasmid #166856)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 166856 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pαH
  • Total vector size (bp) 8331
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS CoV-2 HexaPro full length spike protein
  • Species
    Severe acute respiratory syndrome-related coronavirus
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tags / Fusion Proteins
    • 8xHis (C terminal on insert)
    • Biotinylation site

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid modified and submitted by Sebastiaan Bol, Raphael Reyes, Katie Clarke and Evelien Bunnik. Expression was confirmed by SDS-PAGE and Western blot (anti-His and anti-biotin). Biotin ligase (BirA) and free biotin are required for biotinylation of the biotinylation site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS CoV-2 HexaPro spike (bio-His) was a gift from Evelien Bunnik (Addgene plasmid # 166856 ; http://n2t.net/addgene:166856 ; RRID:Addgene_166856)
  • For your References section:

    SARS-CoV-2 spike-specific memory B cells express higher levels of T-bet and FcRL5 after non-severe COVID-19 as compared to severe disease. Reyes RA, Clarke K, Gonzales SJ, Cantwell AM, Garza R, Catano G, Tragus RE, Patterson TF, Bol S, Bunnik EM. PLoS One. 2021 Dec 22;16(12):e0261656. doi: 10.1371/journal.pone.0261656. eCollection 2021. 10.1371/journal.pone.0261656 PubMed 34936684