XE8 Xwnt-8 myc
(Plasmid #16868)

• Depositing Lab: Randall Moon
• Publication: Christian et al Genes Dev. 1993 Jan. 7(1):13-28.

Backbone

• Vector backbone: SP64T
• Backbone size w/o insert (bp): 3230
• Vector type: Xenopus Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Xwnt-8 myc
• Alt name: xwnt-8
• Alt name: wnt8a
• Species: X. laevis (frog)
• Insert Size (bp): 1470
• Entrez Gene: wnt8a.L (a.k.a. XELAEV_18017109mg, Xwnt8, wnt-8, wnt8, wnt8a, xwnt-8)
• Tag / Fusion Protein:
  • myc

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II, EcoR1 (blunt) (destroyed during cloning)
• 3′ cloning site: Bgl II (destroyed during cloning)
• 5′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Insert: myc epitope insert between aa's 338-339.
Insert size: 1.47 Kb.
Transcription: linearize with BamH1 and transcribe with SP6.

Plasmid History:
Constructed by Jan Christian.

Citation: Christian and Moon, Genes and Development 7, 13 (1993)