|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16935||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK-
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)1200
Entrez Genegsc (a.k.a. ZGSC, ik:tdsubc_1c10, ik:tdsubc_2g4, ik:tdsubc_2h11, xx:tdsubc_1c10, xx:tdsubc_2g4, xx:tdsubc_2h11, zgc:101595)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
Antisense probe cut with EcoR1 and transcribe with T7.
Sense probe cut with Xho1 and transcribe with T3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBluescript goosecoid was a gift from John Postlethwait (Addgene plasmid # 16935 ; http://n2t.net/addgene:16935 ; RRID:Addgene_16935)
For your References section:Goosecoid expression in neurectoderm and mesendoderm is disrupted in zebrafish cyclops gastrulas. Thisse C, Thisse B, Halpern ME, Postlethwait JH. Dev Biol. 1994 Aug . 164(2):420-9. 10.1006/dbio.1994.1212 PubMed 8045345
Map uploaded by the depositor.