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Addgene

IKKε v2-V5
(Plasmid #169770)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 169770 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1 V5-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5523
  • Total vector size (bp) 6807
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2
  • Alt name
    IKKε v2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1971
  • GenBank ID
    NM_001193322
  • Tag / Fusion Protein
    • V5 tag (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IKKε v2-V5 was a gift from Sung-Liang Yu (Addgene plasmid # 169770 ; http://n2t.net/addgene:169770 ; RRID:Addgene_169770)

  • For your References section:

    IKKepsilon isoform switching governs the immune response against EV71 infection. Chang YL, Liao YW, Chen MH, Chang SY, Huang YT, Ho BC, Yu SL. Commun Biol. 2021 Jun 2;4(1):663. doi: 10.1038/s42003-021-02187-x. 10.1038/s42003-021-02187-x PubMed 34079066
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