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ZE33 Prickle-1-GFPnopren-pCS2+
(Plasmid #17074)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 17074 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Zebrafish Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
  • Species
    D. rerio (zebrafish)
  • Mutation
    Pk1GFPnopren deletes the C-terminal six amino acids containing the putative prenylation site.
  • Entrez Gene
    prickle1a (a.k.a. fk71b09, pk1, prickle1, wu:fk71b09)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. The C-terminal prenylation motif KNCIIS was deleted by PCR.

Species of Sequence Origin: zebrafish.

Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp.

Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ZE33 Prickle-1-GFPnopren-pCS2+ was a gift from Randall Moon (Addgene plasmid # 17074 ; ; RRID:Addgene_17074)
  • For your References section:

    Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT. Curr Biol. 2003 Apr 15. 13(8):680-5. 10.1016/S0960-9822(03)00240-9 PubMed 12699626