Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17074||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeZebrafish Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesD. rerio (zebrafish)
MutationPk1GFPnopren deletes the C-terminal six amino acids containing the putative prenylation site.
Entrez Geneprickle1a (a.k.a. fk71b09, pk1, prickle1, wu:fk71b09)
/ Fusion Protein
- GFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer NA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Description: Zebrafish prickle-1, cloned into the vector CS2+GFP-BB, to provide an N-terminal GFP. The C-terminal prenylation motif KNCIIS was deleted by PCR.
Species of Sequence Origin: zebrafish.
Notes for Use: Has internal Not1, Kp1 sites, so linearize with BssH11 and transcribe SP6 for sense RNA. Select clones with amp.
Reference: MT Veeman et al., Current Biology 13: 680-685 (2003).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:ZE33 Prickle-1-GFPnopren-pCS2+ was a gift from Randall Moon (Addgene plasmid # 17074 ; http://n2t.net/addgene:17074 ; RRID:Addgene_17074)