K-SPOTIT1.0
(Plasmid
#171215)
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PurposeExpresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171215 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePLX208
- Backbone size w/o insert (bp) 6778
- Total vector size (bp) 9115
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Modifications to backboneno Hygromycin
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameK-SPOTIT1.0
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SpeciesH. sapiens (human)
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Insert Size (bp)2337
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site MluI (unknown if destroyed)
- 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
- 3′ sequencing primer cacatagcgtaaaaggagcaacatag (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
K-SPOTIT1.0 was a gift from Wenjing Wang (Addgene plasmid # 171215 ; http://n2t.net/addgene:171215 ; RRID:Addgene_171215) -
For your References section:
Designing a Single Protein-Chain Reporter for Opioid Detection at Cellular Resolution. Kroning KE, Wang W. Angew Chem Int Ed Engl. 2021 Mar 4. doi: 10.1002/anie.202101262. 10.1002/anie.202101262 PubMed 33662184
