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pET28a-SUMO-AsCas12f
(Plasmid #171612)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171612 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    AsCas12f1
  • Alt name
    Cas12U3
  • Species
    Other

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that a Q114K mutation was identified in the SUMO tag. This mutation has no effect on the function of the plasmid, as the SUMO tag will be removed during the protein purification procedure.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-SUMO-AsCas12f was a gift from Quanjiang Ji (Addgene plasmid # 171612 ; http://n2t.net/addgene:171612 ; RRID:Addgene_171612)

  • For your References section:

    Programmed genome editing by a miniature CRISPR-Cas12f nuclease. Wu Z, Zhang Y, Yu H, Pan D, Wang Y, Wang Y, Li F, Liu C, Nan H, Chen W, Ji Q. Nat Chem Biol. 2021 Sep 2. pii: 10.1038/s41589-021-00868-6. doi: 10.1038/s41589-021-00868-6. 10.1038/s41589-021-00868-6 PubMed 34475565
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