CBM9-N
(Plasmid #171710)

• Purpose: Expresses SARS-CoV-2 Nucleocapsid protein epitope
• Depositing Lab: Francis Nano
• Publication: McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. doi: 10.1186/s12934-022-01753-0.

Backbone

• Vector backbone: pRSET5A
• Backbone manufacturer: Thermo Fisher
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 3600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): T7 Express lysY/Iq, NEB #C3013I
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SARS-CoV-2 Nucleocapsid
• Insert Size (bp): 728
• GenBank ID: MZ322557
• Entrez Gene: N (a.k.a. GU280_gp10)
• Tag / Fusion Protein:
  • CBM-9 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Esp3I (destroyed during cloning)
• 3′ cloning site: Esp3I (destroyed during cloning)
• 5′ sequencing primer: AAGGTCAGCGTGTTGGTATC or nFseq5A GATCTCGATCCCGCGAAATTAATAC
• 3′ sequencing primer: nRseq5A AAACCCCTCAAGACCCG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA