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pAAV-hSyn1-DIO-ExRai-AKAR2-T/A
(Plasmid #171847)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 171847 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Agilent
  • Backbone size w/o insert (bp) 4068
  • Total vector size (bp) 5286
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow at 30C for a short time to avoid ITR deletions
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ExRai-AKAR2 T/A
  • Alt name
    Excitation-ratiometric A Kinase Activity Reporter 2 Biosensor
  • Species
    Synthetic
  • Insert Size (bp)
    1218
  • Mutation
    T6A phospho-deficient mutation
  • Promoter hSyn1

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn1-DIO-ExRai-AKAR2-T/A was a gift from Richard Huganir (Addgene plasmid # 171847 ; http://n2t.net/addgene:171847 ; RRID:Addgene_171847)

  • For your References section:

    An ultrasensitive biosensor for high-resolution kinase activity imaging in awake mice. Zhang JF, Liu B, Hong I, Mo A, Roth RH, Tenner B, Lin W, Zhang JZ, Molina RS, Drobizhev M, Hughes TE, Tian L, Huganir RL, Mehta S, Zhang J. Nat Chem Biol. 2020 Sep 28. pii: 10.1038/s41589-020-00660-y. doi: 10.1038/s41589-020-00660-y. 10.1038/s41589-020-00660-y PubMed 32989297
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