pcDNA-M45HA
(Plasmid #17573)

• Depositing Lab: Wolfram Brune
• Publication: Mack et al Proc Natl Acad Sci U S A. 2008 Feb 26. 105(8):3094-9.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: murine cytomegalovirus M45 gene
• Alt name: viral inhibitor of RIP1-mediated signaling
• Alt name: vIRS
• Species: murine cytomegalovirus
• Insert Size (bp): 3552
• GenBank ID: DQ978788
• Tag / Fusion Protein:
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry