Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17677||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Gene/Insert namec-src Y416F Y527F
SpeciesG. gallus (chicken)
MutationTyr 416 changed to Phe . Tyr 527 changed to Phe .
Entrez GeneSRC (a.k.a. PP60C-SCR, SDR, c-src)
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Based on plasmid pM5HHB5.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcSrc416/527 was a gift from David Shalloway (Addgene plasmid # 17677 ; http://n2t.net/addgene:17677 ; RRID:Addgene_17677)
For your References section:Activation and suppression of pp60c-src transforming ability by mutation of its primary sites of tyrosine phosphorylation. Kmiecik TE, Shalloway D. Cell. 1987 Apr 10. 49(1):65-73. 10.1016/0092-8674(87)90756-2 PubMed 3103925
Map uploaded by the depositor.