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(Plasmid #31636)


Item Catalog # Description Quantity Price (USD)
Plasmid 31636 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Alt name
  • Species
    O. cuniculus (rabbit)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
  • Promoter cmv
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site vector=XHO1 insert=Sal1 (destroyed during cloning)
  • 5′ sequencing primer gfp-c sequencing
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Make by SS's lab
Randy HAll's HA-RN1 cut with ECOR1 & XHO1
pEGFP-C2.seq cut with ECOR1 & SAL1
eGFP translation starts @ 613 [email protected] 1375

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-rbNHERF1 was a gift from Edward Weinman (Addgene plasmid # 31636 ; ; RRID:Addgene_31636)
  • For your References section:

    Phosphorylation of PDZ1 domain attenuates NHERF-1 binding to cellular targets. Voltz JW, Brush M, Sikes S, Steplock D, Weinman EJ, Shenolikar S. J Biol Chem. 2007 Nov 16. 282(46):33879-87. 10.1074/jbc.M703481200 PubMed 17895247