pEGFP-rbNHERF1
(Plasmid #31636)

• Depositing Lab: Edward Weinman
• Publication: Voltz et al J Biol Chem. 2007 Nov 16. 282(46):33879-87.

Backbone

• Vector backbone: peGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
• Alt name: NHERF-1
• Species: O. cuniculus (rabbit)
• Insert Size (bp): 1090
• GenBank ID: U19815
• Entrez Gene: SLC9A3R1
• Promoter: cmv
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: vector=XHO1 insert=Sal1 (destroyed during cloning)
• 5′ sequencing primer: gfp-c sequencing

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pEGF-RN1.seq
Make by SS's lab
Randy HAll's HA-RN1 cut with ECOR1 & XHO1
pEGFP-C2.seq cut with ECOR1 & SAL1
ligated.
eGFP translation starts @ 613 rN1@ 1375