pEGFP-mKOR
(Plasmid #179285)

• Purpose: Express mouse kappa opioid receptor (GFP tagged) in mammalian cells
• Depositing Lab: Christian Gruber
• Publication: Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055. doi: 10.1021/acs.jmedchem.1c00158. Epub 2021 Jun 23.

Backbone

• Vector backbone: pEGFP-N1
• Total vector size (bp): 5843
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: kappa opioid receptor
• Alt name: KOR
• Species: M. musculus (mouse)
• Insert Size (bp): 1141
• Entrez Gene: Oprk1 (a.k.a. K-OR-1, KOR, KOR-1, MSL-1, Oprk2, R21)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: GAGGTCTATATAAGCAGAGC
• 3′ sequencing primer: ACTTGTGGCCGTTTACGTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry