Purposeanti-Mortalin/GRP75 (Mouse) recombinant mouse monoclonal antibody
|Item||Catalog #||Description||Quantity||Price (USD)|
|Recombinant Antibody||180091-rAb||100 µg of purified recombinant antibody|
|Recombinant Antibody trial size||180091-rAb.T||20 µg of purified recombinant antibody|
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Production & Usage
Production PlasmidAnti-Mortalin/GRP75 [N52A/42.1R]
PurificationPurified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
Storage BufferPBS + 1 mM sodium azide
Terms and Licenses
|Protein||Stress-70 protein, mitochondrial|
|Antigen Description||Identified as off-target mAb in screen for anti-SALM2 NeuroMabs (immunogen was amino acids 551-766 of mouse SALM2). Target identified as Mortalin/GRP75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.|
|Species||M. musculus (mouse)|
|Additional Species Reactivity||H. sapiens (human), R. norvegicus (rat), C. elegans (nematode)|
These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Anti-Mortalin/GRP75 [N52A/42.1R] was a gift from James Trimmer (Addgene antibody # 180091 ; http://n2t.net/addgene:180091 ; RRID:AB_2750800)
For your References section:A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360