pCS2+-dCas9-olEzh2
(Plasmid
#180216)
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PurposeEzh2 fused to dCas9 for H3K27me3 epigenome editing
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180216 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 10618
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameenhancer of zeste 2 polycomb repressive complex 2 subunit
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Alt nameEzh2
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SpeciesOryzias latipes
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Insert Size (bp)6543
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Tags
/ Fusion Proteins
- dCas9 (N terminal on insert)
- FLAG (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+-dCas9-olEzh2 was a gift from Hiroyuki Takeda (Addgene plasmid # 180216 ; http://n2t.net/addgene:180216 ; RRID:Addgene_180216) -
For your References section:
Targeted in vivo epigenome editing of H3K27me3. Fukushima HS, Takeda H, Nakamura R. Epigenetics Chromatin. 2019 Mar 13;12(1):17. doi: 10.1186/s13072-019-0263-z. 10.1186/s13072-019-0263-z PubMed 30871638
Map uploaded by the depositor.
