pAAV-PAG-E2-Crimson-3XNLS-pA
(Plasmid
#183801)
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PurposeAAV vector to express nuclear localized E2-Crimson from PAG promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183801 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE2-Crimson-NLS
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SpeciesOther
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Tag
/ Fusion Protein
- NLS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains only one ITR. This is not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-PAG-E2-Crimson-3XNLS-pA was a gift from Jonathan Ploski (Addgene plasmid # 183801 ; http://n2t.net/addgene:183801 ; RRID:Addgene_183801) -
For your References section:
Adeno-associated viral serotypes differentially transduce inhibitory neurons within the rat amygdala. de Solis CA, Hosek MP, Holehonnur R, Ho A, Banerjee A, Luong JA, Jones LE, Chaturvedi D, Ploski JE. Brain Res. 2017 Oct 1;1672:148-162. doi: 10.1016/j.brainres.2017.07.023. Epub 2017 Jul 29. 10.1016/j.brainres.2017.07.023 PubMed 28764932
