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pGST2-TSG101 (2-145)
(Plasmid #184810)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184810 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGST parallel2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TSG101
  • Species
    H. sapiens (human)
  • Mutation
    aa 2-145 only
  • Entrez Gene
    TSG101 (a.k.a. TSG10, VPS23)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GST-TEVsite-TSG101 (2-145)-stop

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST2-TSG101 (2-145) was a gift from James Hurley (Addgene plasmid # 184810 ; http://n2t.net/addgene:184810 ; RRID:Addgene_184810)

  • For your References section:

    Crystallographic and functional analysis of the ESCRT-I /HIV-1 Gag PTAP interaction. Im YJ, Kuo L, Ren X, Burgos PV, Zhao XZ, Liu F, Burke TR, Bonifacino JS, Freed EO, Hurley JH. Structure. 2010 Nov 10. 18(11):1536-47. 10.1016/j.str.2010.08.010 PubMed 21070952
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