pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_mEGFP
(Plasmid
#187073)
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PurposeMitochondria surface expression of dHaloTag7 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 187073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCDNA5/FR/TO
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTOM20-dHaloTag7
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Alt nameTOM20-dHaloTag7
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Alt namemEGFP
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SpeciesH. sapiens (human)
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MutationdHaloTag7 = HaloTag7-D106A
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_mEGFP was a gift from Kai Johnsson (Addgene plasmid # 187073 ; http://n2t.net/addgene:187073 ; RRID:Addgene_187073) -
For your References section:
Exchangeable HaloTag Ligands for Super-Resolution Fluorescence Microscopy. Kompa J, Bruins J, Glogger M, Wilhelm J, Frei MS, Tarnawski M, D'Este E, Heilemann M, Hiblot J, Johnsson K. J Am Chem Soc. 2023 Feb 8;145(5):3075-3083. doi: 10.1021/jacs.2c11969. Epub 2023 Jan 30. 10.1021/jacs.2c11969 PubMed 36716211