pLX304-EV-IRES-GFP
              
              
                (Plasmid
                
                #187640)
              
            
            
            
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            PurposeEmpty Vector control modified to express GFP
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              Depositing Lab
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          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187640 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepLX304-gate-IRES-GFP
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              Backbone manufacturerGift from V. Koduri
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              Modifications to backboneModified to express GFP
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              Vector typeMammalian Expression, Lentiviral
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                Selectable markersBlasticidin
 
Growth in Bacteria
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            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature30°C
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            Growth Strain(s)NEB Stable
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            Copy numberHigh Copy
 
Gene/Insert
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                Gene/Insert nameEV
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                  Alt nameEmpty Vector
 
Resource Information
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            Article Citing this Plasmid
 
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX304-EV-IRES-GFP was a gift from William Kaelin (Addgene plasmid # 187640 ; http://n2t.net/addgene:187640 ; RRID:Addgene_187640) - 
                
For your References section:
HIF-independent synthetic lethality between CDK4/6 inhibition and VHL loss across species. Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, Perrimon N, Signoretti S, Kaelin WG Jr. Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 10.1126/scisignal.aay0482. 10.1126/scisignal.aay0482 PubMed 31575731