HA-EGFP-NEMO-D304N
(Plasmid #187898)

• Purpose: Mammalian cell expression of D304N mutation in NEMO
• Depositing Lab: Erika Holzbaur
• Publication: Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7. doi: 10.1016/j.molcel.2023.08.005.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 7600
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HA-EGFP-NEMO-D304N
• Alt name: IKBKG
• Species: M. musculus (mouse)
• Insert Size (bp): 2200
• Mutation: D304N
• GenBank ID: AF069542
• Entrez Gene: Ikbkg (a.k.a. 1110037D23Rik, IKK[g], NEMO)
• Promoter: CMV
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Blp1 (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: pd0.5-EGFP-C 5'-GACCACTACCAGCAGAACAC
• 3′ sequencing primer: NA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: E. Laplantine (Institut Pasteur), Tarantino et al. JCB 2014

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.06.21.496850v1 for bioRxiv preprint.