pYBS369
(Plasmid
#18885)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18885 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepYBS317
- Backbone size w/o insert (bp) 11000
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSte7
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)516
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Mutationamino terminal 172 aa of Ste7
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Entrez GeneSTE7 (a.k.a. YDL159W)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer pHybLex_fwd
- 3′ sequencing primer pHybLex-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Has a 0.5kb BamHI-BglII fragmetn from pYEE129 at BamHI of pYBS317. The open reading frame reads through to an unknown stop in the vector.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYBS369 was a gift from Elaine Elion (Addgene plasmid # 18885 ; http://n2t.net/addgene:18885 ; RRID:Addgene_18885) -
For your References section:
Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Choi KY, Satterberg B, Lyons DM, Elion EA. Cell. 1994 Aug 12. 78(3):499-512. 10.1016/0092-8674(94)90427-8 PubMed 8062390