POLG-pcDNA3.1
(Plasmid #191487)

• Purpose: Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
• Depositing Lab: Hans Zempel
• Publication: Mammalian expression plasmid with POLG gene (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 9173
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: POLG
• Species: H. sapiens (human)
• Insert Size (bp): 3720
• Entrez Gene: POLG (a.k.a. MDP1, MIRAS, MTDPS4A, MTDPS4B, PEO, POLG1, POLGA, SANDO, SCAE)
• Promoter: CMV, T7
• Tag / Fusion Protein:
  • V5 tag, 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAGGG
• 3′ sequencing primer: TCTAGATTCAATGGTCCAGGCTGGCTTCG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry