EC64422
(Plasmid
#191768)
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PurposeCRISPR mutagenesis in Medicago truncatula
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191768 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonen/a
- Backbone size w/o insert (bp) 4897
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Vector typePlant Expression, CRISPR
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9
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Alt namegRNA to be added by user
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SpeciesStreptococcus pyogenes
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Insert Size (bp)9419
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
User inserts own guides for CRISPR in Medicago truncatula
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EC64422 was a gift from Wendy Harwood (Addgene plasmid # 191768 ; http://n2t.net/addgene:191768 ; RRID:Addgene_191768) -
For your References section:
Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method. Lawrenson T, Atkinson N, Forner M, Harwood W. Methods Mol Biol. 2023;2653:221-252. doi: 10.1007/978-1-0716-3131-7_15. 10.1007/978-1-0716-3131-7_15 PubMed 36995630
