pELPS-eDHFR-YFP-T2A-Renilla
(Plasmid #193253)

• Purpose: Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
• Depositing Lab: Mark Sellmyer
• Publication: Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 10.1016/j.ymthe.2019.10.007. Epub 2019 Oct 15.

Backbone

• Vector backbone: pELPS
• Backbone size w/o insert (bp): 6770
• Total vector size (bp): 8975
• Modifications to backbone: BamHI and SalI for inserts.
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: LB or TB for growth
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase
• Alt name: eDHFR-YFP-T2A-Renilla
• Species: the Escherichia coli
• Insert Size (bp): 2205
• Mutation: None
• GenBank ID: WP_000624375.1
• Promoter: EF1a
• Tags / Fusion Proteins:
  • yellow fluorescent protein (YFP) (C terminal on insert)
  • Renilla luciferase (rLuc) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CTTGGTTCATTCTCAAGCCTCAGAC
• 3′ sequencing primer: GCAATAGCATGATACAAAGGCATTAAAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry