lentiCRISPR.v2.TetON_Cas13d_U6_DR
(Plasmid
#196727)
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PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196727 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonelentiCRISPR v2 (#52961)
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Backbone manufacturerFeng Zhang Lab
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Vector typeLentiviral, CRISPR
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Selectable markersPuromycin ; miRFP670nano
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRfxCas13d-T2A-miRFP670nano
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Alt namertTA-advanced-P2A-PuroR
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SpeciesRuminococcus flavefaciens XPD3003
- Promoter TRE
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Tags
/ Fusion Proteins
- Nucleoplasmin NLS (N terminal on insert)
- SV40 NLS (C terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lentiCRISPR.v2.TetON_Cas13d_U6_DR was a gift from Bernhard Schmierer (Addgene plasmid # 196727 ; http://n2t.net/addgene:196727 ; RRID:Addgene_196727)
