pFastBac flag Mel18
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1968||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4775
Vector typeInsect Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GenePcgf2 (a.k.a. Mel1, Mel18, Rnf1, Rnf110, Zfp14, Zfp144, mel-1, mel-18)
/ Fusion Protein
- flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Polyhedrin forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
mouse Mel-18 cDNA with N-terminal Flag fusion. (kingston #1385)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFastBac flag Mel18 was a gift from Robert Kingston (Addgene plasmid # 1968 ; http://n2t.net/addgene:1968 ; RRID:Addgene_1968)
For your References section:Propagation of silencing; recruitment and repression of naive chromatin in trans by polycomb repressed chromatin. Lavigne M, Francis NJ, King IF, Kingston RE. Mol Cell 2004 Feb 13;13(3):415-25. 10.1016/S1097-2765(04)00006-1 PubMed 14967148
Map uploaded by the depositor.