His-huH3-huH4
(Plasmid
#197272)
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PurposeHuman (hu) histones H3 and H4 inserted into a plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 197272 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCDF
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namehuH3
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SpeciesH. sapiens (human)
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Tags
/ Fusion Proteins
- Histidine tag (N terminal on backbone)
- No tag
Gene/Insert 2
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Gene/Insert namehuH4
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- No tag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
His-huH3-huH4 was a gift from Mark Glover (Addgene plasmid # 197272 ; http://n2t.net/addgene:197272 ; RRID:Addgene_197272) -
For your References section:
Structural insights into gammaH2Ax containing nucleosomes. Panigrahi R, Edwards R, Islam MT, Lu J, Kulepa A, Kim TH, Mark Glover JN. Nucleic Acids Res. 2025 Oct 14;53(19):gkaf1028. doi: 10.1093/nar/gkaf1028. 10.1093/nar/gkaf1028 PubMed 41123210