NF-155 pcDNA3
(Plasmid
#197290)
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PurposePlasmid expressing an antigen targeting the Plasmid expressing an antigen targeting Neurofascin-155
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Depositing Lab
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Depositing OrganizationUniversity of Edinburgh
Located in United Kingdom of Great Britain and Northern Ireland -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197290 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 197290-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 197290-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneUnknown
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePan-Neurofascin (extracellular)
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SpeciesR. norvegicus (rat)
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Entrez GeneNfasc (a.k.a. NF)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a validated antigen plasmid of Anti-Pan-Neurofascin (extracellular) [A12/18R].
Additional reference: https://pubmed.ncbi.nlm.nih.gov/10931875/.
DNA (Catalog # 197290-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 197290-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NF-155 pcDNA3 was a gift from Peter Brophy (Addgene plasmid # 197290 ; http://n2t.net/addgene:197290 ; RRID:Addgene_197290) -
For your References section:
Transient expression of neurofascin by oligodendrocytes at the onset of myelinogenesis: implications for mechanisms of axon-glial interaction. Collinson JM, Marshall D, Gillespie CS, Brophy PJ. Glia. 1998 May;23(1):11-23 10.1002/(sici)1098-1136(199805)23:1<11::aid-glia2>3.0.co;2-7 PubMed 9562181