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pRSETA-betaFlincG
(Plasmid #19736)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 19736 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSET-A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha, 37C, overnight
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    beta-FlincG
  • Alt name
    FlincG
  • Species
    Bovine
  • Insert Size (bp)
    2800
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSETA-betaFlincG was a gift from Wolfgang Dostmann (Addgene plasmid # 19736 ; http://n2t.net/addgene:19736 ; RRID:Addgene_19736)
  • For your References section:

    Differential patterning of cGMP in vascular smooth muscle cells revealed by single GFP-linked biosensors. Nausch LW, Ledoux J, Bonev AD, Nelson MT, Dostmann WR. Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):365-70. 10.1073/pnas.0710387105 PubMed 18165313