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(Plasmid #199559)


Item Catalog # Description Quantity Price (USD)
Plasmid 199559 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2580
  • Total vector size (bp) 4073
  • Vector type
    GATEWAY-compatible entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy


  • Gene/Insert name
    Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites
  • Alt name
  • Alt name
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    MIR390a (a.k.a. AT2G38325, MICRORNA 390, MIR390, MIR390A, microRNA390A, p_MI0001000)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-BS-AtMIR390a-B/c was a gift from Alberto Carbonell (Addgene plasmid # 199559 ; ; RRID:Addgene_199559)
  • For your References section:

    Transgene-free, virus-based gene silencing in plants by artificial microRNAs derived from minimal precursors. Cisneros AE, Martin-Garcia T, Primc A, Kuziuta W, Sanchez-Vicente J, Aragones V, Daros JA, Carbonell A. Nucleic Acids Res. 2023 Oct 27;51(19):10719-10736. doi: 10.1093/nar/gkad747. 10.1093/nar/gkad747 PubMed 37713607