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pAAVS1-Puro-PECAM1-Neo
(Plasmid #201054)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 201054 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2874
  • Total vector size (bp) 15082
  • Vector type
    AAV
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Adeno-associated virus integration site 1
  • Alt name
    AAVS1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    12208
  • Entrez Gene
    AAVS1 (a.k.a. AAV)
  • Promoter PECAM1
  • Tag / Fusion Protein
    • Neomycin resistance (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site SbfI (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    PECAM-eGFP was a gift from Victoria Bautch (Addgene plasmid # 14688 ; http://n2t.net/addgene:14688 ; RRID:Addgene_14688). pAAVS1-P-CAG-DEST was a gift from Knut Woltjen (Addgene plasmid # 80490 ; http://n2t.net/addgene:80490 ; RRID:Addgene_80490).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

1. The VEGF receptor flt-1 (VEGFR-1) is a positive modulator of vascular sprout formation and branching morphogenesis. Kearney JB, Kappas NC, Ellerstrom C, DiPaola FW, Bautch VL. Blood. 2004 Jun 15. 103(12):4527-35. 10.1182/blood-2003-07-2315 PubMed 14982871
2. Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives. Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K. Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012. 10.1016/j.ymeth.2015.12.012 PubMed 26707206

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS1-Puro-PECAM1-Neo was a gift from Paul Burridge (Addgene plasmid # 201054 ; http://n2t.net/addgene:201054 ; RRID:Addgene_201054)

  • For your References section:

    Nilotinib-induced alterations in endothelial cell function recapitulate clinical vascular phenotypes independent of ABL1. Pinheiro EA, DeKeyser JM, Lenny B, Sapkota Y, Burridge PW. Sci Rep. 2024 Mar 26;14(1):7123. doi: 10.1038/s41598-024-57686-8. 10.1038/s41598-024-57686-8 PubMed 38532120
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