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(Plasmid #201922)


Item Catalog # Description Quantity Price (USD)
Plasmid 201922 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Total vector size (bp) 9754
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Propagate with 0.5% (w/v) glucose
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
    Methanococcus maripaludis
  • Insert Size (bp)
  • Mutation
    E412P, E414F, T417K, P495M, I496W, F529S
  • GenBank ID
  • Promoter GlnS (constitutive)
  • Tag / Fusion Protein
    • None

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Sep-tRNA v2.0
  • Species
    Methanococcus maripaludis
  • Insert Size (bp)
  • Promoter lpp (constitutive)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
  • Species
    E. coli
  • Insert Size (bp)
  • Promoter OXB20 (constitutive)

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
  • Species
    E. coli
  • Insert Size (bp)
  • Mutation
    H67R , E216N, D217G, F219Y, T229S, N274W
  • Promoter tac (lactose/IPTG inducible)

Cloning Information for Gene/Insert 4

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid should be paired with pCDF-Frb-v1 (#201923, which enables the biosynthesis of non-hydrolyzable phosphoserine) and a compatible plasmid expressing protein of interest (e.g. #174076 for a sfGFP-150TAG control) in the cell line BL21(DE3) deltaSerC (#197656). This system is used to translationally incorporate non-hydrolyzable phosphoserine into target proteins at TAG amber stop codons in E coli.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pERM2-nhpSer was a gift from Richard Cooley & Ryan Mehl (Addgene plasmid # 201922 ; ; RRID:Addgene_201922)
  • For your References section:

    Autonomous Synthesis of Functional, Permanently Phosphorylated Proteins for Defining the Interactome of Monomeric 14-3-3zeta. Zhu P, Stanisheuski S, Franklin R, Vogel A, Vesely CH, Reardon P, Sluchanko NN, Beckman JS, Karplus PA, Mehl RA, Cooley RB. ACS Cent Sci. 2023 Apr 10;9(4):816-835. doi: 10.1021/acscentsci.3c00191. eCollection 2023 Apr 26. 10.1021/acscentsci.3c00191 PubMed 37122473