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Addgene

pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA
(Plasmid #204547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204547 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-IRES-puro-3xFLAG
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NBR1
  • Species
    H. sapiens (human)
  • Mutation
    deltaUBA
  • Entrez Gene
    NBR1 (a.k.a. 1A1-3B, IAI3B, M17S2, MIG19)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA was a gift from Masaaki Komatsu (Addgene plasmid # 204547 ; http://n2t.net/addgene:204547 ; RRID:Addgene_204547)

  • For your References section:

    Integrated proteomics identifies p62-dependent selective autophagy of the supramolecular vault complex. Kurusu R, Fujimoto Y, Morishita H, Noshiro D, Takada S, Yamano K, Tanaka H, Arai R, Kageyama S, Funakoshi T, Komatsu-Hirota S, Taka H, Kazuno S, Miura Y, Koike M, Wakai T, Waguri S, Noda NN, Komatsu M. Dev Cell. 2023 May 9:S1534-5807(23)00191-0. doi: 10.1016/j.devcel.2023.04.015. 10.1016/j.devcel.2023.04.015 PubMed 37192622
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