pBS-MXT GIRK2
(Plasmid #20675)

• Depositing Lab: Henry Lester
• Publication: Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-8.

Backbone

• Vector backbone: pBluescript II KS (-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3224
• Vector type: Xenopus expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mus musculus G protien coupled inward rectifier K+ channel 2
• Alt name: GIRK 2
• Alt name: Kcnj6
• Species: M. musculus (mouse)
• Insert Size (bp): 1300
• Mutation: A369V
• GenBank ID: NP_034736.2
• Entrez Gene: Kcnj6 (a.k.a. BIR1, GIRK2, KATP2, KCNJ7, Kir3.2, weaver, wv)
• Tags / Fusion Proteins:
  • 5' UTR Xenopus B-globin (N terminal on backbone)
  • 3' UTR Xenopus B- globin (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Spe I (destroyed during cloning)
• 3′ cloning site: Spe I (destroyed during cloning)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GIRK 2 was obtained originally from L. Salkoff's lab

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry