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Addgene

pTrc_AraC-S170-LOV
(Plasmid #206805)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 206805 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTKEI-Dest modified
  • Backbone size w/o insert (bp) 3656
  • Total vector size (bp) 4961
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AraC-AsLOV2 fusion
  • Species
    E. coli, A. sativa
  • Insert Size (bp)
    1305
  • Tag / Fusion Protein
    • His-tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTrc_AraC-S170-LOV was a gift from Dominik Niopek (Addgene plasmid # 206805 ; http://n2t.net/addgene:206805 ; RRID:Addgene_206805)
  • For your References section:

    Dissecting the Determinants of Domain Insertion Tolerance and Allostery in Proteins. Mathony J, Aschenbrenner S, Becker P, Niopek D. Adv Sci (Weinh). 2023 Oct;10(28):e2303496. doi: 10.1002/advs.202303496. Epub 2023 Aug 10. 10.1002/advs.202303496 PubMed 37562980