pcDNA3 GFP GFP PTEN
(Plasmid
#20739)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 20739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3-GFP-GFP
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePTEN
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SpeciesH. sapiens (human)
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Insert Size (bp)1900
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GenBank IDNM_000314
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Entrez GenePTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)
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Tag
/ Fusion Protein
- GFP-GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer BGH rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GFP-PTEN was cloned into pcDNA3 as two fragments. The enzyme/insert order is: HindIII - GFP - BamHI - PTEN - EcoRI. Next, pcDNA3 GFP-GFP-PTEN was prepared by inserting GFP into the HindIII site of pcDNA3-GFP-PTEN.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 GFP GFP PTEN was a gift from Alonzo Ross (Addgene plasmid # 20739) -
For your References section:
PTEN enters the nucleus by diffusion. Liu F, Wagner S, Campbell RB, Nickerson JA, Schiffer CA, Ross AH. J Cell Biochem. 2005 Oct 1. 96(2):221-34. 10.1002/jcb.20525 PubMed 16088943