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PB-TET-MKOS
(Plasmid #20959)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20959 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 20959-D50 50 μg of DNA in Tris buffer $495
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DNA 20959-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    PB-TET
  • Backbone size w/o insert (bp) 9186
  • Vector type
    Mammalian Expression ; piggyBac
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cMyc, KLF4, Oct4, Sox2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5046
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)
  • Entrez Gene
    Klf4 (a.k.a. EZF, Gklf, Zie)
  • Entrez Gene
    Pou5f1 (a.k.a. NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
  • Entrez Gene
    Sox2 (a.k.a. Sox-2, lcc, ysb)
  • Tag / Fusion Protein
    • IRES-bGeo (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site attB1 (not destroyed)
  • 3′ cloning site attB2 (not destroyed)
  • 5′ sequencing primer M13 F, T7
  • 3′ sequencing primer M13 R, T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    MKOS liked by 2A peptides obtained from Keisuke Kaji et al (Nature. 2009 Mar 1) piggyBac terminal repeats obtained from Pentao Liu at Sanger.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional reference, Kaji et al Nature. 2009 Mar 1

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TET-MKOS was a gift from Andras Nagy (Addgene plasmid # 20959 ; http://n2t.net/addgene:20959 ; RRID:Addgene_20959)

  • For your References section:

    piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A. Nature. 2009 Apr 9;458(7239):766-70. doi: 10.1038/nature07863 10.1038/nature07863 PubMed 19252478
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