Halo-DNAPKcs
(Plasmid
#210787)
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PurposePlasmid for expression of HaloTag-DNA-PKcs in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210787 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDNA-PKcs
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SpeciesH. sapiens (human)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG-HaloTag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer bGH PolyA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Halo-DNAPKcs was a gift from Jens Schmidt (Addgene plasmid # 210787 ; http://n2t.net/addgene:210787 ; RRID:Addgene_210787) -
For your References section:
Single-molecule imaging reveals the kinetics of non-homologous end-joining in living cells. Mikhova M, Goff NJ, Janovic T, Heyza JR, Meek K, Schmidt JC. Nat Commun. 2024 Nov 23;15(1):10159. doi: 10.1038/s41467-024-54545-y. 10.1038/s41467-024-54545-y PubMed 39578493