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Addgene

pHAGE2-Ef1a-SOX17E57D-3xFLAG
(Plasmid #216199)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216199 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE2-EF1a
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOX17
  • Species
    H. sapiens (human)
  • Mutation
    Glu113Glu (silent mutation); Glu120Glu (silent mutation); Glu122Asp
  • Entrez Gene
    SOX17 (a.k.a. VUR3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE2-Ef1a-SOX17E57D-3xFLAG was a gift from Ralf Jauch (Addgene plasmid # 216199 ; http://n2t.net/addgene:216199 ; RRID:Addgene_216199)

  • For your References section:

    An acidic residue within the OCT4 dimerization interface of SOX17 is necessary and sufficient to overcome its pluripotency-inducing activity. Ho SY, Hu H, Ho DHH, Renom APS, Yeung SW, Boerner F, Weng M, Hutchins AP, Jauch R. Stem Cell Reports. 2025 Mar 11;20(3):102398. doi: 10.1016/j.stemcr.2025.102398. Epub 2025 Feb 6. 10.1016/j.stemcr.2025.102398 PubMed 39919754
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