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pGP-RSET-iGABASnFR2-6xHis
(Plasmid #218885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218885 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRSET
  • Backbone manufacturer
    ThermoFisher Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iGABASnFR2
  • Alt name
    variant 4409
  • Species
    Pseudomonas fluorescens, A. victoria (jellyfish)
  • Insert Size (bp)
    1710
  • Mutation
    S99A F102Y F104Y L178S
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.03.25.644953 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGP-RSET-iGABASnFR2-6xHis was a gift from GENIE Project (Addgene plasmid # 218885 ; http://n2t.net/addgene:218885 ; RRID:Addgene_218885)

  • For your References section:

    iGABASnFR2 is an improved genetically encoded protein sensor of GABA. Kolb I, Hasseman JP, Matsumoto A, Jensen TP, Kopach O, Arthur BJ, Zhang Y, Tsang A, Reep D, Tsegaye G, Zheng J, Patel RH, Looger LL, Marvin JS, Korff WL, Rusakov DA, Yonehara K, Turner GC. eLife. 2026 Mar 18;14:RP108319. doi: 10.7554/eLife.108319. 10.7554/eLife.108319 PubMed 41848771
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