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Addgene

pCMV-Flag-hGSDMD
(Plasmid #218938)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218938 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human GSDMD
  • Alt name
    gasdermin D
  • Species
    H. sapiens (human)
  • Entrez Gene
    GSDMD (a.k.a. DF5L, DFNA5L, FKSG10, GSDMDC1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Flag-hGSDMD was a gift from Christopher LaRock (Addgene plasmid # 218938 ; http://n2t.net/addgene:218938 ; RRID:Addgene_218938)

  • For your References section:

    Group A Streptococcus induces GSDMA-dependent pyroptosis in keratinocytes. LaRock DL, Johnson AF, Wilde S, Sands JS, Monteiro MP, LaRock CN. Nature. 2022 May;605(7910):527-531. doi: 10.1038/s41586-022-04717-x. Epub 2022 May 11. 10.1038/s41586-022-04717-x PubMed 35545676
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