pYPQ230-sbcB-N
(Plasmid
#220290)
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PurposeGateway entry plasmid (attL1& attR5) expressing sbcB exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220290 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepYPQ230
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Vector typePlant Expression, CRISPR ; Gateway compatible sbcB-XTEN linker- LbCas12a entry clone
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesbcB-XTEN linker-LbCas12a
Cloning Information
- Cloning method Other
- 5′ sequencing primer gtaaaacgacggccag
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYPQ230-sbcB-N was a gift from Yiping Qi (Addgene plasmid # 220290 ; http://n2t.net/addgene:220290 ; RRID:Addgene_220290) -
For your References section:
Increasing Cas12a- and Cas9-mediated deletion sizes with exonucleases. Wang X, Clem J, Malzahn A, Qi Y. Plant Physiol. 2025 Oct 31;199(3):kiaf507. doi: 10.1093/plphys/kiaf507. 10.1093/plphys/kiaf507 PubMed 41182408
