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Addgene

plenti hSyn GAPDH
(Plasmid #220918)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 220918 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLV-hSyn
  • Backbone size w/o insert (bp) 8462
  • Total vector size (bp) 9470
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Bleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GAPDH
  • Alt name
    glyceraldehyde-3-phosphate dehydrogenase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1008
  • Entrez Gene
    GAPDH (a.k.a. G3PD, GAPD, HEL-S-162eP)
  • Promoter hSyn

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer hSyn-F
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    plenti hSyn GAPDH was a gift from Timothy Ryan (Addgene plasmid # 220918 ; http://n2t.net/addgene:220918 ; RRID:Addgene_220918)