Tg(Sox17::EMTB-3xGFP)
(Plasmid #221190)

• Purpose: To specifically label microtubules (MTs) in Kupffer's Vesicle (KV)
• Depositing Lab: Heidi Hehnly
• Publication: Wu et al Development. 2025 May 15;152(10):dev204687. doi: 10.1242/dev.204687. Epub 2025 May 19.

Backbone

• Vector backbone: Custom Tol2 backbone (pUC57 with modified Tol2 R & L arms)
• Backbone manufacturer: Nemametrix, Inc (dba InVivo Biosystems)
• Backbone size w/o insert (bp): 3599
• Total vector size (bp): 12265
• Modifications to backbone: None
• Vector type: Zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Ensconsin Microtubule-Binding Protein
• Alt name: EMTB
• Species: H. sapiens (human)
• Insert Size (bp): 2256
• Mutation: Microtubule binding-domain of ensconsin (A.A. 18-283)
• GenBank ID: LT734165.1
• Entrez Gene: MAP7 (a.k.a. E-MAP-115, EMAP115)
• Promoter: Sox17
• Tag / Fusion Protein:
  • 3 EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13R
• 3′ sequencing primer: M13F

Resource Information

• Supplemental Documents:
  • hheh02-sox17emtb-3xegfp-in-tol2.gb
  • Sox17-EMTB-GFP in tol2 Authentication Report

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.12.593765 for bioRxiv preprint.